The protective effect of Jangkanghwan (Korean traditional food) on lipopolysaccharide-induced disruption of the colonic epithelial barrier

Abstract Jangkanghwan (JKH) is a Korean traditional food that mixture of ingredients and medicine ingredients, it has been observed to produce satisfactory anti-inflammatory, antioxidant, antibacterial effects. In the current study, JKH was administered by gavage BALB/C mice with lipopolysaccharide (LPS)-induced colonic epithelial dysfunction, mouse body weight intake were recorded. Indexes such as paracellular permeability, serum inflammatory cytokines, bacterial translocation used comprehensively evaluate regulatory effect on function, qPCR Western blot also analyze expression tight junction (TJ)-related genes, occludin, claudin, zonula occludens (ZOs) proteins, adhesion molecules (JAM) in tissue. The experimental results indicated relieved edema liver, spleen, mesenteric lymph node tissues, reduced loss appetite diarrhea caused LPS injection mice. It increased amount from 3.7 g/day group 4.7 g per day; water content feces 13.86% less than group. response LPS, protected integrity colon, permeability fluorescent macromolecules one-fourth group, enhanced mRNA protein TJ-related proteins colon Our findings highlight benefits intestinal health relieving systemic inflammation, relevant aspects its use functional food.